CD81 antibody

Catalog No. ABIN4888503

This Mouse Monoclonal antibody specifically detects CD81 in FACS, WB, IP, Func, IHC (p) and ICC. It exhibits reactivity toward Human, Rabbit and Cat.

Quick Overview for CD81 antibody (ABIN4888503)

Target: CD81

Reactivity: Human, Rabbit, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD81 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Clone: M38

Product Details anti-CD81 Antibody

Purpose: Anti-Hu CD81 Purified Low Endotoxin

Specificity: The antibody M38 reacts with an extracellular epitope of CD81, a 25 kDa member of the tetraspanin family, expressed on majority of cells.

Cross-Reactivity (Details): Human, Feline (Cat), Rabbit

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Endotoxin Level: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: MOLT-4 (human T-ALL cell line)

Isotype: IgG1

Application Details

Application Notes: Functional application: In human MOLT-4 T-cell line the antibody M38 inhibits syncytium formation induced by coculture with human T-cell leukemia virus type 1 (HTLV-1)-positive human T-cell lines.
Flow cytometry: recommended dilution: 1 μg/mL.
Western blotting: recommended dilution: 1-2 μg/mL, positive control: Jurkat cells, non-reducing conditions.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for CD81

Target: CD81

Alternative Name: CD81

Background: CD81 Molecule,CD81 (TAPA-1), a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T and other cells, e.g. in muscles CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus.,S5.7, CVID6, TAPA1, TSPAN28

Gene ID: 975

UniProt: P60033

Pathways: Inositol Metabolic Process, Hepatitis C